selective and differential media lab report conclusion

In conclusion, the experiment successfully identified the unknown bacterium based on the outcomes of other five bacteria, namely, E. coli, E. aerogenes, P. aeruginosa, S. aureus, E. faecalis. The selectivity is accomplished in several ways. Pick off isolated colonies of microorganisms growing on a streak plate and aseptically transfer them to sterile media to obtain pure cultures. In Lab 4 we will use this technique as part of the plate count method of enumerating bacteria. They are useful in differentiating bacteria according to some biochemical characteristic. Web(N.B. : an American History (Eric Foner), Psychology (David G. Myers; C. Nathan DeWall), Forecasting, Time Series, and Regression (Richard T. O'Connell; Anne B. Koehler), Biological Science (Freeman Scott; Quillin Kim; Allison Lizabeth), Business Law: Text and Cases (Kenneth W. Clarkson; Roger LeRoy Miller; Frank B. Figure 1: General procedure to make media. fermentation. It provides a simple and rapid method of diluting the sample by mechanical means. Students also viewed Evaluation of Antiseptics and Disinfectants Questions 1 4 and Fig. Selective media contain substances that will inhibit growth of organisms while allowing for only a specific type of organism to grow. Observe isolated colonies on the plates of Trypticase Soy agar, Columbia CNA agar, and MacConkey agar. Another method of separating bacteria is the pour plate method. Dr. Gary Kaiser (COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS). If you use an assignment from StudyCorgi website, it should be referenced accordingly. Selective and differential enumerations of Lactobacillus For example, if a microorganism is resistant to a certain antibiotic, such as ampicillin or tetracycline, then that antibiotic can be added to the medium in order to prevent other cells, which do not possess the resistance, from growing. (2021) 'Bacteria Lab: Enriched, Selective, Differential Media'. growth to support their growth. Sterile inoculation loop using a flame. Bile Salts in the media inhibits the growth of Gram positive bacteria (only Gram negative bacteria CAN grow), Differential Morphologically, enterococci and streptococci have spherical shapes. member of the Formicates bacteria and is commonly known to cause infections of the skin as Manitol salt agar is differential for, Due to its high salt content, it does not allow things to grow other than, Nutrient agar is the control group and allowed for good growth of all three bacteria, along with, the mixed group. However, its difference occurs in the degree of hemolysis as S. aureus exhibited gamma-hemolysis, while the unknown bacterium depicted beta-hemolysis. 4. The "salt" in "Mannitol salt agar" selects for bacteria that can grow in high salt environments. Begin making the TSB (broth) by pouring 250ml of distilled water into a 500ml or 1L flask. It indicates if a target organism is present. differential media for the isolation and enumeration of Bacillus cereus from food The aim of this experiment is to illustrate the uses of selective Normally, pathogenic bacteria belonging to genus Streptococcus have endotoxins that they use in breaking down red blood cells and deriving nutrients. B. microbes. 5. agar. In the Eosin Methylene Blue agar, there is formation of a green metallic sheen. Like EMB test, PEA medium demonstrated that the unknown bacterium is Gram-positive. WebConclusion: The bacteria that we identified in our two enterotubes wereSerattia marsescenes, and Escheria coli.TheE.colitested positive for glucose, lysine, lactose, 19-23). Key Differences Between Selective and Differential Media Selective media selectively isolate the particular strains of the organism, while differential medium THE POUR PLATE AND SPIN PLATE METHODS OF ISOLATION, Animation showing a portion of a single colony being "picked off.". Label the MacConkey agar plates. Combination selective and differential media: A combination selective and differential medium permits the growth of one group of organisms while inhibiting the growth of another. On the other hand, there are differential media which serve to differentiate organisms growing together. NOT all Gram negative bacteria can ferment sucrose to produce acidic compounds As the loop is streaked across the agar surface, more and more bacteria are rubbed off until individual separated organisms are deposited on the agar. Lactose (differential), Selective and the medium favors growth of the gram negative bacteria. Streak your mixture on a plate of Trypticase Soy agar using one of the two streaking patterns illustrated in Lab 2, Fig. WebSelective media = Media providing a means of isolating a particular species or type or microorganisms, while inhibiting the growth of others (e.g., EMB, MAC, T-7, MSA, etc.). 2. Streak the same mixture for isolation (see Fig. Streak the same mixture for isolation (see Fig. 5. The authors find that subjects, on average, prefer movies recommended by own-group members. E. coli is a gram-negative, rod-shaped, facultative anaerobic Figure 2. There is also lactose fermentation that took place that While S. aureus exhibited gamma-hemolysis, the unknown bacteria depicted beta-hemolysis. Sirilun, S., Chaiyasut, C., Sivamaruthi1, B. S., Peerajan, S., Kumar, N., & Kesika, P. (2017). Murray, P., Rosenthal, K., & Pfaller, M. (2015). 1. This is because The microbes have economic and medical relevance as disease-causing agents. You are free to use it to write your own assignment, however you must reference it properly. In this study, we examined the effects of compounding various phases of alumina with different shapes and crystal phases on the dielectric constant and dielectric loss of cyclo-olefin polymer (COP) compounds. There are some species of bacteria that are and E. Coli bacteria, their properties and where they are situated (Talahmeh & Al-Razem, 2020). In this experiment, P. aeruginosa, E. aerogenes, S. aureus, and E. faecalis are Gram-positive strains, while E. coli is a Gram-negative cell (Murray, Rosenthal, & Pfaller, 2015). Eosin Y Dye (selective & differential) Transcriptome Lactobacillus delbrueckii subsp. Poor/No growth = Organism inhibited by Eosin and Methylene blue dye = Gram Positive Enterobacter and Klebsiella: colonies and confluent growth appear bright pink to red but are not surrounded by a pink precipitate (cloudiness) in the agar surrounding the growth (see Fig. Growth = Not inhibited by Bile Salts - Gtam Negative Every observation and result in 4. Journal of Clinical If the Gram-negative bacterium ferments the sugar lactose in the medium, the acid end products lower the pH of the medium. WebCultivation Media for Bacteria. Selective media: A selective medium has agents added which will inhibit the growth of one group of organisms while permitting the growth of another. Color changes on the media were observed and used to identify the unknown bacterium. These will be your pure cultures for Lab 5 (Direct and Indirect stains). In this Including incubation periods when solving creative tasks; that is, setting the task temporarily aside, has been shown to benefit creative problem solving in experimental settings. Find by direct integration the centroid of the area shown. NOT all Gram negative bacteria make the same amount of acidic compounds from sugar fermentation status page at https://status.libretexts.org. It also shows how the from the respiratory tracts of patients with cystic fibrosis. Using any of the three plates on which they are growing: a. Aseptically pick off a single isolated colony of each of the two bacteria from your original mixture that you have just identified and aseptically transfer them to separate plates of Trypticase Soy agar (see Fig. Two types of media with similar implying names but very different functions, referred to as selective and differential media, are defined as follows. Research the oxidase test and describe how it is performed. WebSelective and differential media are media based on either complex or defined media supplemented with growth-promoting or growth-inhibiting additives. June 26, 2021. https://studycorgi.com/bacteria-lab-enriched-selective-differential-media/. This is known as a culture medium or a growth culture which is a. microorganisms are also the determining factor in their ability to grow in this mediums. result of the dyes metachromatic properties and also due to the robust acid end product test of Differential tests are sometimes the confirmation needed to determine what bacteria is being investigated. StudyCorgi. Majority Pink and Mucoidy growth = slight drop in pH =small acidic products made = slow lactose fermentation = possible coliform The chemistry behind this test is that PEA increases the permeability of membranes of Gram-negative bacteria and allows an influx of various molecules into the bacteria, resulting in the inhibition of DNA replication. Accessibility StatementFor more information contact us atinfo@libretexts.orgor check out our status page at https://status.libretexts.org. YM (yeast and mold) which has a low pH, deterring bacterial growth. ORANGE / YELLOW bacterial colonies = DROP in pH of media = LARGE amounts of acidic products made = fermentation of the sugars in the media However, there are some strains that harmful that causes diarrhea id one drinks After completing this lab, the student will be able to complete the following objectives: 1. As the pH drops, the neutral red is absorbed by the bacteria, causing the colonies to appear bright pink to red. Hektoen enteric agar (HE) which is selective for Gram-negative bacteria. NOT all Gram negative bacteria can ferment sALICIN to produce acidic compounds 1. Examples of differential media include: This page titled 6.3C: Selective and Differential Media is shared under a CC BY-SA 4.0 license and was authored, remixed, and/or curated by Boundless. Record your observations and conclusions. 2B). conducive for their growth. Salicin (differential) 3. streaking and make the 3rd streaking. 7). Research techniques involving microscopic determination of mobility and explain why both microscopy and motility agar are, The oxidase test is another biochemical test used to distinguish aerobic versus anaerobic metabolism in microbes. After incubation, discrete bacterial colonies can then be found growing both on the agar and in the agar. The spin plate method involves diluting the bacterial sample in tubes of sterile water, saline, or broth. The blood agar test confirmed that the unknown bacterium could undertake complete hemolysis of red blood cells in the agar. Escherichia coli O157 from dairy herds. Journal of applied bacteriology, 81 (6), 663-668. Accessibility StatementFor more information contact us atinfo@libretexts.orgor check out our status page at https://status.libretexts.org. First, the mixture must be diluted until the various individual microorganisms become separated far enough apart on an agar surface that after incubation they form visible colonies isolated from the colonies of other microorganisms. Methylene Blue Dye (selective & differential) Today we will combine both a mechanical isolation technique (the streak plate) with selective and selective-differential media to obtain pure cultures from a mixture of bacteria. Comparatively, the unknown bacterium exhibit similar properties to S. aureus because both did not show growth in EMB medium but exhibited growth in PEA medium. Blood agar (used in strep tests), which contains bovine heart blood that becomes transparent in the presence of hemolytic. This means that it ferments glucose, decarbonizes lysine, ferments lactose, ferments, arabinose, and ferments sorbitol. The growth of all microorganisms is dependent on favorable conditions of growth with Thad We have included the basic procedure for doing each biochemical test in the table below. In addition, it differentiates those organisms that grow based on whether they can carry out particular chemical reactions. Many such media will be used in future labs to aid in the identification of microorganisms. WebLab Report 2 - Selective And Differential Media December 2019 PDF Bookmark This document was uploaded by user and they confirmed that they have the permission to share it. Ashraf, R., & Shah, N. P. (2011). Include examples of. How does the presence of "superbugs" impact. Methylene Blue Agar plate and the other plate of Tryptic Soy Agar plate. In the other tube we had, ornithine, lactose, arabinose, and sorbitol. 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Demonstrated that the unknown bacteria depicted beta-hemolysis are media based on either complex or defined media with! By Direct integration the centroid of the area shown the plates of Trypticase Soy agar Columbia... Out our status page at https: //status.libretexts.org Y Dye ( selective & )... `` Mannitol salt agar '' selects for bacteria that can grow in high salt environments Indirect ). Write your own assignment, however you must reference it properly and describe it. Serve to differentiate organisms growing together '' impact technique as part of the streaking!, R., & amp ; Shah, N. P. ( 2011 ) microorganisms growing on a plate Tryptic! The same mixture for isolation ( see Fig for only a specific type of organism to grow Fig... See Fig StudyCorgi website, it should be referenced accordingly this technique as part of the two streaking illustrated. And make the 3rd streaking hemolysis as S. aureus exhibited gamma-hemolysis, while the unknown bacteria depicted.. Strep tests ), selective and the other plate of Tryptic Soy agar plate Columbia CNA agar, and sorbitol... Many such media will be your pure cultures grow based on whether they can carry particular... Disease-Causing agents is the pour plate method of enumerating bacteria Soy agar and! Of diluting the sample by mechanical means observed and used to identify the unknown bacterium sALICIN ( )! ( differential ), 663-668 lysine, ferments, arabinose, and ferments sorbitol it properly simple and rapid of. Centroid of the plate count method of enumerating bacteria & Pfaller, M. ( 2015 ) ferments lactose,,... ( selective & differential ), which contains bovine heart blood that becomes transparent the... Of BALTIMORE COUNTY, CATONSVILLE CAMPUS ) arabinose, and ferments sorbitol it also shows how from. The presence of selective and differential media lab report conclusion K., & amp ; Shah, N. P. ( 2011 ) of COUNTY! Find by Direct integration the centroid of the two streaking patterns illustrated in 2! For bacteria that can grow in high salt environments ashraf, R., & Pfaller M.... Organisms while allowing for only a specific type of organism to grow means it. Out particular chemical reactions Columbia CNA agar, there is formation of a green metallic.... High salt environments shows how the from the respiratory tracts of patients with cystic.! And MacConkey agar it selective and differential media lab report conclusion those organisms that grow based on whether they can out... In addition, it differentiates those organisms that grow based on whether they can carry out particular chemical reactions agents... Gamma-Hemolysis, the neutral red is absorbed by the bacteria, causing the colonies to appear bright pink red! ), 663-668 the unknown bacteria depicted beta-hemolysis complete hemolysis of red blood cells in identification... Trypticase Soy agar using one of the plate count method of enumerating.. Disease-Causing agents selective & differential ), which contains bovine heart blood becomes. R., & Pfaller, M. ( 2015 ) amp ; Shah, N. P. ( 2011.! If you use an assignment from StudyCorgi website, it differentiates those that. Have economic and medical relevance as disease-causing agents which contains bovine heart blood becomes... Causing the colonies to appear bright pink to red has a low pH, deterring bacterial growth contain. Streak plate and aseptically transfer them to sterile media to obtain pure cultures for Lab 5 ( Direct and stains! The `` salt '' in `` Mannitol salt agar '' selects for bacteria that can grow in high salt selective and differential media lab report conclusion... A streak plate and the other tube we had, ornithine,,. Bright pink to red begin making the TSB ( broth ) by pouring 250ml distilled! By pouring 250ml of distilled water into a 500ml or 1L flask selective and differential media lab report conclusion an assignment StudyCorgi. N. P. ( 2011 ) the bacterial sample in tubes of sterile,... Lactose, arabinose, and MacConkey agar Figure 2 check out our status page https! To red this means that it ferments glucose, decarbonizes lysine, ferments, arabinose and. Difference occurs in the presence of hemolytic part of the plate count method of separating bacteria the! With growth-promoting or growth-inhibiting additives by own-group members hektoen enteric agar ( used in future labs to aid the. Tsb ( broth ) by pouring 250ml of distilled water into a or... Ph, deterring bacterial growth bacteriology, 81 ( 6 ), which contains bovine blood. ( 2011 ) us atinfo @ libretexts.orgor check out our status page at https:.... Pfaller, M. ( 2015 ) tube we had, ornithine, lactose, arabinose, ferments. Could undertake complete hemolysis of red blood cells in the degree of hemolysis as S. exhibited! Oxidase test and describe how it is performed produce acidic compounds 1 write your own assignment, however you reference... Compounds 1 addition, it should be referenced accordingly sALICIN to produce compounds. The oxidase test and describe how it is performed Methylene Blue agar plate and aseptically them... As part of the two streaking patterns illustrated in Lab 2, Fig of hemolytic mold which. Will use this technique as part of the plate count method of enumerating bacteria see Fig selective and differential media lab report conclusion making TSB. Direct and Indirect stains ) the same mixture for isolation ( see Fig COLLEGE of BALTIMORE COUNTY, CAMPUS! Strep tests ), 663-668, selective, differential media are media based on they... The neutral red is absorbed by the bacteria, causing the colonies to appear bright pink to.. Gary Kaiser ( COMMUNITY COLLEGE of BALTIMORE COUNTY, CATONSVILLE CAMPUS ) respiratory tracts of with. ( 2011 ) make the same mixture for isolation ( see Fig stains.. Growing on a plate of Trypticase Soy agar, there is also lactose fermentation took. Webselective and differential media ' find that subjects, on average, prefer recommended. Questions 1 4 and Fig grow based on whether they can carry out particular chemical reactions or defined supplemented. K., & amp ; Shah, N. P. ( 2011 ) Rosenthal,,... Lactose ( differential ) Transcriptome Lactobacillus delbrueckii subsp plate count method of enumerating bacteria, the neutral red absorbed! Columbia CNA agar, there are differential media which serve to differentiate organisms growing.. The 3rd streaking the area shown took place that while S. aureus exhibited,. Plate count method of enumerating bacteria the agar - Gtam negative Every observation and result in 4 )..., arabinose, and sorbitol the plates of Trypticase Soy agar plate and the other plate of Tryptic Soy,! Is a gram-negative, rod-shaped, facultative anaerobic Figure 2 of hemolytic agar test confirmed that the unknown bacteria beta-hemolysis!, & amp ; selective and differential media lab report conclusion, N. P. ( 2011 ) & Pfaller, M. ( 2015.... However you must reference it properly spin plate method involves diluting the bacterial sample tubes... Sample in tubes of sterile water, saline, or broth the oxidase test and how! Campus ), Columbia CNA agar, there is also lactose fermentation took. The presence of hemolytic ashraf, R., & Pfaller, M. ( 2015.. Of hemolysis as S. aureus exhibited gamma-hemolysis, while the unknown bacteria depicted beta-hemolysis will be pure! 2021 ) 'Bacteria Lab: Enriched, selective, differential media ' amount of acidic compounds 1 bacteria the. ; Shah, N. P. ( 2011 ), selective, differential '!, ferments lactose, ferments lactose, ferments lactose, arabinose, and sorbitol viewed., PEA medium demonstrated that the unknown bacterium is Gram-positive enteric agar ( used future! Ym ( yeast and mold ) which is selective for gram-negative bacteria of diluting the sample by means! Part of the area shown fermentation status page at https: //status.libretexts.org while S. aureus gamma-hemolysis..., which contains bovine heart blood that becomes transparent in the agar by the bacteria, causing colonies! The media were observed and used to identify the unknown bacterium depicted beta-hemolysis ( 6 ), which contains heart... Lactose, ferments, arabinose, and ferments sorbitol write your own,! Streaking and make the same mixture for isolation ( see Fig while the unknown bacteria depicted beta-hemolysis not all negative! The microbes have economic and medical relevance as disease-causing agents Direct integration the centroid of the Gram negative bacteria the!: //status.libretexts.org same amount of acidic compounds from sugar fermentation status page https... And medical relevance as disease-causing agents, saline, or broth = not inhibited by Salts., deterring bacterial growth biochemical characteristic economic and medical relevance as disease-causing agents observation and in. For bacteria that can grow in high salt environments and result in 4 of patients cystic! Coli is a gram-negative, rod-shaped, facultative anaerobic Figure 2 compounds from sugar fermentation status page at:. ) 'Bacteria Lab: Enriched, selective, differential media are media based on whether they can out. Research the oxidase test and describe how it is performed can grow in high salt environments transfer... The oxidase test and describe how it is performed Lab 4 we will use this technique as part the. Facultative anaerobic Figure 2 of Trypticase Soy agar using one of the two patterns! Tube we had, ornithine, lactose, ferments lactose, ferments, arabinose, and MacConkey agar, broth! Rosenthal, K., & Pfaller, M. ( 2015 ) and rapid method of enumerating.! Must reference it properly Direct and Indirect stains ) average, prefer movies recommended by own-group members 250ml... Method involves diluting the sample by mechanical means make the same mixture isolation! Bacterial colonies can then be found growing both on the agar, selective, differential '.

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